In situ hybridization, fluorescent
An important molecular cytogenetic method for identifying chromosomes and parts of chromosomes, deciphering chromosome rearrangements, and locating genes on chromosomes.
“Fluorescent” means emitting light that comes from a reaction within the emitter. “In situ” refers to the fact that this techniques is done with the chromosomes, cells or tissue in place (in situ) on a microscope slide.
In situ hybridization is a technique in which single-stranded nucleic acids (DNA or RNA) are permitted to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. Through nucleic acid hybridization, the degree of sequence identity can be determined and specific sequences detected and sometimes those sequences can be located on a specific chromosome. A nucleic acid labelled with a fluorescent dye is hybridized to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localization of genes to specific chromosomes.
Fluorescent in situ hybridization is abbreviated FISH.
Read Also:
- In situ hybridization
The use of a DNA or RNA probe to detect complementary genetic material in cells or tissue. In situ hybridization involves hybridizing a labeled nucleic acid to suitably prepared cells or tissues on microscope slides to allow visualization in situ (in the normal location).
- In situ
In the normal location. An in situ tumor is one that is confined to its site of origin and has not invaded neighboring tissue or gone elsewhere in the body. For example, squamous cell carcinoma in situ is an early stage of skin cancer.
- Inherited immunodeficiency disease
Primary immunodeficiency disease.
- Inherited metabolic diseases
the presence of uric acid in the urine of people with gout).
- Inhibin
One of two hormones (designated inhibin-A and inhibin-B) secreted by the gonads (by Sertoli cells in the male and the granulosa cells in the female) and that inhibit the production of follicle-stimulating hormone (FSH) by the pituitary gland. The inhibins are also involved in the control of the production of gametes and embryonic and fetal […]