Sequence tagged site (STS)
A short (200 to 500 base pair) DNA sequence that occurs but once in the genome and whose location and base sequence are known.
STSs are detectable by polymerase chain reaction (PCR), are useful for localizing and orienting the mapping and sequence data, and serve as landmarks on the physical map of a genome.
Expressed sequence tags (ESTs) are STSs derived from cDNAs (complementary DNAs).
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- Sequence, complementary
Nucleic acid sequence of bases that can form a double- stranded structure by matching base pairs. For example, the complementary sequence to C-A-T-G (where each letter stands for one of the bases in DNA) is G-T-A-C.
- Sequence, conserved
A base sequence in a DNA molecule (or an amino acid sequence in a protein) that has remained essentially unchanged throughout evolution.
- Sequence, draft DNA
Sequence of a DNA with less accuracy than a finished sequence. In a draft sequence, some segments are missing or are in the wrong order or are oriented incorrectly. A draft sequence is as opposed to a finished DNA sequence.
- Sequence, finished DNA
A DNA sequence in which the bases are identified to an accuracy of no more than 1 error in 10,000 and are placed in the right order and orientation along a chromosome with almost no gaps. A finished sequence is as opposed to a draft DNA sequence.
- Sequence, intervening
An intervening sequence is the part of a gene that is initially transcribed from the DNA into RNA (specifically, into the primary RNA transcript) but then is excised (removed) from it when the so-called exxon sequences on either side of it are spliced together. Intervening sequences intervene between the exxons. Intervening sequences are also called […]